Wednesday, November 10, 2010

Verification Procedure for Reactive Molecules in ASEA™

I've gotten a few request for this.
Follow the link to view the pdf (adobe reader is required).
https://extranet.securefreedom.com/Asea/Personal/Resources/ReactiveMoleculeVerification%20-%20Update%20Jun%202010.pdf
Here's an excerpt from the paper:
The reactive molecules in ASEA™ are produced by a complex proprietary electrochemical process that reduces and oxidizes the base saline solution, resulting in an equilibrium of several known reactive molecules. These reactive molecules are stable in ASEA™ and measurable using standard analytic methods. Such reactive molecules are the same as those that are naturally produced inside of living cells and have been successfully measured over the last 30 years by the use of certain fluorescent dyes that act as indicators. Verification of the reactive molecules in ASEA™ is done regularly by utilizing three of these same standard fluorescent indicators, namely R-Phycoerythrin (RPE), Aminophenyl fluorescein (APF) and Hydroxyphenyl fluorescein (HPF). These fluorescent molecules physically change shape (and brightness) when they come into contact with specific reactive molecules. These conformal changes enhance or reduce the fluorescence of the indicators at certain given frequencies of light. This change in fluorescence is then measured using a fluorospectrometer that measures the intensity of the spectrum of light emitted from the indicators.
Fig 1: Description of the three indicators used to measure the concentration of reactive molecules in ASEA™. The table on the bottom lists some of the fundamental properties for R-PE.
Description of Indicators:
The two new novel probes, Aminophenyl fluorescein (APF) and Hydroxyphenyl fluorescein (HPF) developed by Tetsuo Nagano et. al. (1), are selective for the detection of highly reactive oxygen species (hROS). Both probes have little reactivity towards other forms of ROS.
Assay Principle: Conformal changes in APF and HPF molecules change fluorescent properties.
Phycobiliprotein Fluorescent Dye Spectral Properties.
Fluor
Molecular Weight
Excitation Max (nm)
Emission Max (nm)
R-Phycoerythrin

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